A Note on T H E Non-specific Action of So-called

نویسنده

  • H. J. PHELPS
چکیده

Rats were immunized ( 1 ) with Jensen rat sarcoma tissue; ( 2 ) with emulsions of normal rat spleen; ( 3 ) with normal rat blood. Sera from rats of these three series were tested against ( a ) cultures of Jensen rat sarcoma cells; ( b ) cultures of normal spleen cells; (c) cultures of mouse carcinoma 63; ( d ) cultures of cells of normal mouse spleen. Jensen Rat Sarcoma Tissue as Antigen: Two series of '( tumour-resistant " rats were employed. ( 1 ) Rats of the Jensen rat sarcoma susceptible strain of the Imperial Cancer Research Fund Laboratories. These rats had been used for routine transplantation of the Jensen rat sarcoma; the implants had grown for a time and had then regressed. ( 2 ) Rats of the Wistar strain in which the Jensen rat sarcoma cells do not flourish readily. Twelve such rats were given routine inoculations with minced Jensen rat sarcoma. Of these, 9 showed transient growth of a tumour; one produced a good tumour of progressive growth which was subsequently used for further transplantation; two rats died, one after a small growth had entirely regressed. Two series each of six rats were selected for immunization with Jensen rat sarcoma. One series consisted of resistant rats of the J.R.S. susceptible strain arid one of Wistar rats which had been given a test inoculation. All these rats were given intraperitoneal injections of 1 C.C. of minced J.R.S. weekly. Normal Spleen as Antigen: A series of 6 normal Wistar rats were similarly injected weekly with doses of 1 C.C. of minced spleen obtained from normal rats of various strains. Normal Blood as Antigen: Six Balogh rats were injected weekly with 1 C.C. each of citrated whole blood from normal rats, usually the same rats as were used to provide spleen tissue.

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تاریخ انتشار 2010